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FLT3 antibody (FITC)

FLT3 Reactivity: Human FACS Host: Mouse Monoclonal BV10A4 FITC
Catalog No. ABIN1302921
  • Target See all FLT3 Antibodies
    FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))
    Reactivity
    • 167
    • 53
    • 7
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 170
    • 13
    • 2
    • 1
    Mouse
    Clonality
    • 171
    • 15
    Monoclonal
    Conjugate
    • 70
    • 15
    • 12
    • 11
    • 9
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    This FLT3 antibody is conjugated to FITC
    Application
    • 131
    • 79
    • 54
    • 54
    • 34
    • 26
    • 19
    • 14
    • 9
    • 8
    • 4
    • 3
    • 2
    • 1
    • 1
    Flow Cytometry (FACS)
    Specificity
    The mouse monoclonal antibody BV10A4 (BV10) reacts with an extracellular epitope of CD135 (FLT3, FLK2, STK-1), a 130-160 kDa type I transmembrane receptor tyrosine kinase that is involved in early steps of hematopoiesis.
    No Cross-Reactivity
    Mouse
    Cross-Reactivity (Details)
    Human
    Purification
    Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    Immunogen
    BV-173 leukemic cell line
    Clone
    BV10A4
    Isotype
    IgG1
    Top Product
    Discover our top product FLT3 Primary Antibody
  • Application Notes
    Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
    Comment

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

    Restrictions
    For Research Use only
  • Buffer
    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Haylock, Horsfall, Dowse, Ramshaw, Niutta, Protopsaltis, Peng, Burrell, Rappold, Buhring, Simmons: "Increased recruitment of hematopoietic progenitor cells underlies the ex vivo expansion potential of FLT3 ligand." in: Blood, Vol. 90, Issue 6, pp. 2260-72, (1997) (PubMed).

  • Target
    FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))
    Alternative Name
    CD135 (FLT3 Products)
    Synonyms
    FLT3 antibody, B230315G04 antibody, CD135 antibody, Flk-2 antibody, Flk2 antibody, Flt-3 antibody, Ly72 antibody, wmfl antibody, FLK-2 antibody, FLK2 antibody, STK1 antibody, fms related tyrosine kinase 3 antibody, FMS-like tyrosine kinase 3 antibody, fms-related tyrosine kinase 3 antibody, FLT3 antibody, Flt3 antibody
    Background
    Fms related tyrosine kinase 3,CD135 / FLT3, also known as FLK2 or STK-1 is a receptor tyrosine kinase that plays important roles in hematopoiesis. After binding of Flt3 ligand (FL), CD135 homodimerizes and stimulates proliferation, differentiation and protects the cell from apoptosis. The loss of CD90 and gain of CD135 expression marks the loss of self-renewal in hematopoietic stem cell population. Detectable CD135 expression appears first at low levels on the surface of primitive multilineage progenitor cells and disappears during defined stages of B-cell development, but is upregulated and maintained during maturation of monocytes. CD135 is also expressed on thymocytes, dendritic cell progenitors and on mature dendritic cells, as well as on various malignant hematopoietic cells.,FLT3, FLK2, STK1
    Gene ID
    2322
    UniProt
    P36888
    Pathways
    RTK Signaling
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