Periostin antibody (AA 577-810)

Catalog No. ABIN5519054

Product Details anti-Periostin Antibody

Target: Periostin (POSTN)

Binding Specificity: AA 577-810

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Periostin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: Anti-Periostin/Postn Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Periostin/Postn Antibody Picoband® (ABIN5519054). Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived rat Periostin recombinant protein (Position: K577-Q810).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Gillan, L., Matei, D., Fishman, D. A., Gerbin, C. S., Karlan, B. Y., Chang, D. D. Periostin secreted by epithelial ovarian carcinoma is a ligand for alpha-V-beta-3 and alpha-V-beta-5 integrins and promotes cell motility. Cancer Res. 62: 5358-5364, 2002. 2. Kuhn, B., del Monte, F., Hajjar, R. J., Chang, Y.-S., Lebeche, D., Arab, S., Keating, M. T. Periostin induces proliferation of differentiated cardiomyocytes and promotes cardiac repair. Nature Med. 13: 962-969, 2007. 3. Shao, R., Bao, S., Bai, X., Blanchette, C., Anderson, R. M., Dang, T., Gishizky, M. L., Marks, J. R., Wang, X.-F. Acquired expression of periostin by human breast cancers promotes tumor angiogenesis through up-regulation of vascular endothelial growth factor receptor 2 expression. Molec. Cell. Biol. 24: 3992-4003, 2004.

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Periostin

Target: Periostin (POSTN)

Alternative Name: Postn (POSTN Products)

Background:

Synonyms: Periostin ,Periostin, osteoblast specific factor (Predicted), isoform CRA_a ,Postn ,Postn_predicted ,rCG_49960 ,

Tissue Specificity: Ubiquitous. Highly expressed in testis.

Background: POSTN (Periostin), also known as PN or OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. It was found that purified recombinant PN supported adhesion of ovarian epithelial cells. And periostin was overexpressed by the majority of human primary breast cancers examined. Transfected tumor cell lines overexpressing periostin showed accelerated growth and angiogenesis as xenografts in immunocompromised animals. It was showed that extracellular periostin induced reentry of differentiated mammalian cardiomyocytes into the cell cycle.

Molecular Weight: 93 kDa

Gene ID: 361945