PD-L1 antibody

Catalog No. ABIN5526896

This Mouse Monoclonal antibody specifically detects PD-L1 in FACS and IHC (fro). It exhibits reactivity toward Human and Non-Human Primate.

Quick Overview for PD-L1 antibody (ABIN5526896)

Target: PD-L1 (CD274 (PD-L1))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This PD-L1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 29E-2A3

Product Details anti-PD-L1 Antibody

Purpose: Anti-Hu CD274 Purified

Specificity: The mouse monoclonal antibody 29E.2A3 recognizes an extracellular epitope of CD274 / PD-L1 (also known as B7-H1), a 40 kDa type I transmembrane protein expressed by dendritic cells, activated T cells, activated monocytes, and in various tissues, above all in heart and skeletal muscle, placenta and lung, and in many cancer cells, including T cell lymphomas, melanomas, and glioblastomas.

Cross-Reactivity (Details): Human, Non-Human Primates

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Full length human CD274

Isotype: IgG2b kappa

Application Details

Application Notes: Immunohistochemistry (frozen sections): Acetone fixation.
Flow cytometry: Recommended dilution: 1-4 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: CD274

Background: CD274 Molecule,CD274 / PD-L1 (programmed death ligand-1), also known as B7-H1, is a member of the B7 family of regulatory proteins. It can act as both costimulatory and coinhibitory molecule for T cells. Interaction with its receptor CD279 (PD1) appears to be important in the maintenance of peripheral tolerance and in prevention of tumor rejection. Even pathogens (e.g. Schistosoma) may exploit CD274 to evade an immune response. Besides CD279, existence of other receptor(s) for CD274 is likely.,B7H1, PDL1, PDCD1L1, PDCD1LG1, PDCD1 ligand 1, B7-H1

Gene ID: 29126

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints