ARNT antibody (AA 513-544)

Catalog No. ABIN5530297

This anti-ARNT antibody is a Rabbit Polyclonal antibody detecting ARNT in WB and IF. Suitable for Human.

Quick Overview for ARNT antibody (AA 513-544) (ABIN5530297)

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARNT antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-ARNT Antibody

Binding Specificity: AA 513-544

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This ARNT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513-544 amino acids from the Central region of human ARNT.

Isotype: Ig Fraction

Application Details

Application Notes: For IF starting dilution is: 1:10~50

For WB starting dilution is: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ARNT

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Alternative Name: ARNT

Background: The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1. A t(1,12)(q21,p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia. Alternative splicing results in multiple transcript variants.

Molecular Weight: 87 kDa

Gene ID: 405

UniProt: P27540

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect