Nucleolin antibody (AA 276-304)

Catalog No. ABIN5531758

This anti-Nucleolin antibody is a Rabbit Polyclonal antibody detecting Nucleolin in WB and IF. Suitable for Human.

Quick Overview for Nucleolin antibody (AA 276-304) (ABIN5531758)

Target: Nucleolin (NCL)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nucleolin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-Nucleolin Antibody

Binding Specificity: AA 276-304

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This NCL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-304 amino acids from the Central region of human NCL.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: NCL

Background: Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

Molecular Weight: 77 kDa

Gene ID: 4691

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization