PKC iota antibody (N-Term)

Catalog No. ABIN5531853

The Rabbit Polyclonal anti-PKC iota antibody is suitable to detect PKC iota in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for PKC iota antibody (N-Term) (ABIN5531853)

Target: PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC iota antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PKC iota Antibody

Binding Specificity: AA 176-207, N-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Immunogen: This PKC iota antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-207 amino acids from the N-terminal region of human PKC iota.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.1 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for PKC iota

Target: PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))

Alternative Name: PKC iota

Background: PKC iota belongs to the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehydes-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis.

Molecular Weight: 68 kDa

Gene ID: 5584

UniProt: P41743

Pathways: Neurotrophin Signaling Pathway, Cell-Cell Junction Organization, Tube Formation