Transglutaminase antibody (AA 429-458)

Catalog No. ABIN5532299

This Rabbit Polyclonal antibody specifically detects Transglutaminase in WB, IHC (p) and IF. It exhibits reactivity toward Human.

Quick Overview for Transglutaminase antibody (AA 429-458) (ABIN5532299)

Target: Transglutaminase

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Transglutaminase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-Transglutaminase Antibody

Binding Specificity: AA 429-458

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Immunogen: This Transglutaminase (TGM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 429-458 amino acids from the Central region of human Transglutaminase (TGM2).

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Transglutaminase

Target: Transglutaminase

Background: Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. TGM2 acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. It has been identified as the autoantigen implicated in celiac disease.

Molecular Weight: 77 kDa

Gene ID: 7052

UniProt: P21980