Pyruvate Kinase antibody (C-Term)

Catalog No. ABIN5532984

The Rabbit Polyclonal anti-Pyruvate Kinase antibody is suitable to detect Pyruvate Kinase in samples from Human, Mouse, Rat and Monkey. It has been validated for WB, IF and IHC (p).

Quick Overview for Pyruvate Kinase antibody (C-Term) (ABIN5532984)

Target: Pyruvate Kinase (PK)

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Pyruvate Kinase antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Pyruvate Kinase Antibody

Binding Specificity: AA 476-505, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Immunogen: This Pyruvate Kinase (PKM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-505 amino acids from the C-terminal region of human Pyruvate Kinase (PKM2).

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

For IF starting dilution is: 1:200

For IHC-P starting dilution is: 1:50~1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Pyruvate Kinase

Target: Pyruvate Kinase (PK)

Alternative Name: Pyruvate Kinase

Background: There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.

Molecular Weight: 58 kDa

Gene ID: 5315

UniProt: P14618