MED16 antibody (AA 253-281)

Catalog No. ABIN5533370

Product Details anti-MED16 Antibody

Target: MED16 (Mediator Complex Subunit 16 (MED16))

Binding Specificity: AA 253-281

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED16 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This MED16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-281 amino acids from the Central region of human MED16.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for MED16

Target: MED16 (Mediator Complex Subunit 16 (MED16))

Alternative Name: MED16 (MED16 Products)

Background: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Molecular Weight: 97 kDa

Gene ID: 10025

UniProt: Q9Y2X0

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha