USP20 antibody (AA 310-339)

Catalog No. ABIN5534787

The Rabbit Polyclonal anti-USP20 antibody has been validated for WB. It is suitable to detect USP20 in samples from Human and Mouse.

Quick Overview for USP20 antibody (AA 310-339) (ABIN5534787)

Target: USP20 (Ubiquitin Specific Peptidase 20 (USP20))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP20 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-USP20 Antibody

Binding Specificity: AA 310-339

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This USP20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 310-339 amino acids from the Central region of human USP20.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for USP20

Target: USP20 (Ubiquitin Specific Peptidase 20 (USP20))

Alternative Name: USP20

Background: Deubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'-and 'Lys-63'-linked polyubiquitin chains.

Molecular Weight: 102 kDa

Gene ID: 10868

UniProt: Q9Y2K6

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling