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DGKA antibody (C-Term)

The Rabbit Polyclonal anti-DGKA antibody has been validated for WB and IHC (p). It is suitable to detect DGKA in samples from Human.
Catalog No. ABIN5536026

Quick Overview for DGKA antibody (C-Term) (ABIN5536026)

Target

See all DGKA Antibodies
DGKA (Diacylglycerol Kinase, alpha 80kDa (DGKA))

Reactivity

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Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This DGKA antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Binding Specificity

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    AA 598-628, C-Term

    Purification

    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

    Immunogen

    This DGKA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 598-628 amino acids from the C-terminal region of human DGKA.

    Isotype

    Ig Fraction
  • Application Notes

    For WB starting dilution is: 1:1000

    For IHC-P starting dilution is: 1:50~100

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    2 mg/mL

    Buffer

    Supplied in PBS with 0.09 % (W/V) sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
  • Target

    DGKA (Diacylglycerol Kinase, alpha 80kDa (DGKA))

    Alternative Name

    DGKA

    Background

    Upon cell stimulation, the kinase DGKA converts the second messenger diacylglycerol into phophatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity. DGKA is stimulated by calcium and phosphatidylserine, and is phosphorylated by protein kinase C. Tissue expression is in lymphocytes and oligodengroglial cells. DGKA contains 2 zinc-dependent phorbol-ester and DAG binding domains, and 2 EF-hand calcium binding domains.

    Molecular Weight

    83 kDa

    Gene ID

    1606

    UniProt

    P23743
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