LIN28B antibody (AA 95-128)

Catalog No. ABIN5536364

This Rabbit Polyclonal antibody specifically detects LIN28B in WB, FACS, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for LIN28B antibody (AA 95-128) (ABIN5536364)

Target: LIN28B (Lin-28 Homolog B (LIN28B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIN28B antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LIN28B Antibody

Binding Specificity: AA 95-128

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This LIN28B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-128 amino acids from the Central region of human LIN28B.

Isotype: Ig Fraction

Application Details

Application Notes: For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.33 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for LIN28B

Target: LIN28B (Lin-28 Homolog B (LIN28B))

Alternative Name: LIN28B

Background: Lin-28 homolog B (LIN28B) is overexpressed in hepatocellular carcinoma. The heterochronic gene lin-28 is a key regulator of developmental timing in the nematode Caenorhabditis elegans. Similar with lin-28 proteins, LIN28B conserves a cold shock domain and a pair of CCHC zinc finger domains. Phylogenetic analysis suggests that they might arise as a result of duplication from an ancestral gene. Overexpression of LIN28B was noted in most HCC cell lines and clinical samples. A short LIN28B isoform was also identified in non-tumor liver tissue and fetal liver. Although predominantly localized in the cytoplasm, LIN28B protein shows cell cycle-dependent nuclear translocation in Huh7 cells. Induced expression of exogenous LIN28B in a tet-off cell line promoted cancer cell proliferation.

Molecular Weight: 27 kDa

Gene ID: 389421

UniProt: Q6ZN17