RUVBL1 antibody (AA 247-280)

Catalog No. ABIN5537474

This Rabbit Polyclonal antibody specifically detects RUVBL1 in WB, FACS and IHC (p). It exhibits reactivity toward Human.

Quick Overview for RUVBL1 antibody (AA 247-280) (ABIN5537474)

Target: RUVBL1 (RuvB-Like 1 (E. Coli) (RUVBL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUVBL1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RUVBL1 Antibody

Binding Specificity: AA 247-280

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Immunogen: This RUVBL1 (TIP49) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 247-280 amino acids from the Central region of human RUVBL1 (TIP49).

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.35 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for RUVBL1

Target: RUVBL1 (RuvB-Like 1 (E. Coli) (RUVBL1))

Alternative Name: RUVBL1

Background: RUVBL1 possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. It is a component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1 -CTNNB1 complex. High levels of autoantibodies against RUVBL1 are detected in sera of patients with autoimmune diseases such as polymyositis/dermatomyosistis and autoimmune hepatitis.

Molecular Weight: 50 kDa

Gene ID: 8607

UniProt: Q9Y265

Pathways: Telomere Maintenance