NSG1 antibody (N-Term)

Catalog No. ABIN5539461

The Goat Polyclonal anti-NSG1 antibody has been validated for WB, ELISA and ICC. It is suitable to detect NSG1 in samples from Mouse and Rat. There is 1 publication available.

Quick Overview for NSG1 antibody (N-Term) (ABIN5539461)

Target: NSG1 (D4S234E) (Neuron-Specific Protein Family Member 1 (D4S234E))

Reactivity: Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This NSG1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Grade: Verified

Product Details anti-NSG1 Antibody

Binding Specificity: N-Term

Purpose: Nsg1 / Neep21

Sequence: EKGTKQPLLE DGFD

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 22 kDa band observed in Mouse fetal Brain lysates (calculated MW of 20.9 kDa according to NP_035072.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunocytochemistry: Primary DIV9 cells from Hippocampus E18 Rat embryos, stained for neuronal marker MAP2 in blue and for glial marker GFAP in green showed exclusive labelling of EB11637 (red) in the neurons only. Recommended concentrat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-NSG1 antibody (ABIN5539461)

Digilio, Yap, Winckler et al.: "Ctip2-, Satb2-, Prox1-, and GAD65-Expressing Neurons in Rat Cultures: Preponderance of Single- and Double-Positive Cells, and Cell Type-Specific Expression of Neuron-Specific Gene Family Members, ..." in: PLoS ONE, Vol. 10, Issue 10, pp. e0140010, (2015) (PubMed).

Target Details for NSG1

Target: NSG1 (D4S234E) (Neuron-Specific Protein Family Member 1 (D4S234E))

Alternative Name: Nsg1

Background: Nsg1, neuron specific gene family member 1, Bsmrb, Neep21, PEP1, brain neuron cytoplasmic protein 1/2, brain specific mRNA b, neuron-specific protein family member 1

Gene ID: 27065, 18196, 25247

NCBI Accession: NP_077042