PI15 antibody (AA 128-141)

Catalog No. ABIN5539462

This anti-PI15 antibody is a Goat Polyclonal antibody detecting PI15 in WB, IHC and ELISA. Suitable for Human.

Quick Overview for PI15 antibody (AA 128-141) (ABIN5539462)

Target: PI15 (Peptidase Inhibitor 15 (PI15))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PI15 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-PI15 Antibody

Binding Specificity: AA 128-141

Purpose: PI15 (aa128-141)

Sequence: RTGRYRSILQ LVKP

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5 μg/mL.

Western Blot: Approx 33 kDa band observed in lysates of cell line HeLa (calculated MW of 29.1 kDa according to NP_056970.1). Recommended concentration: 0.3-1 μg/mL. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.

Peptide ELISA: antibody detection limit dilution 1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PI15

Target: PI15 (Peptidase Inhibitor 15 (PI15))

Alternative Name: PI15

Background: PI15, peptidase inhibitor 15, CRISP8, DKFZp686F0366, P24TI, P25TI, 25 kDa trypsin inhibitor, CRISP-8, PI-15, cysteine-rich secretory protein 8, protease inhibitor 15, sugarCrisp

Gene ID: 51050, 94227, 301489

NCBI Accession: NP_056970