ALPL antibody (AA 42-53)

Catalog No. ABIN5539470

The Goat Polyclonal anti-ALPL antibody has been validated for WB and ELISA. It is suitable to detect ALPL in samples from Human.

Quick Overview for ALPL antibody (AA 42-53) (ABIN5539470)

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ALPL antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-ALPL Antibody

Binding Specificity: AA 42-53

Purpose: ALPL (aa42-53)

Sequence: ELQKLNTNVA KN

Specificity: This antibody is expected to recognize isoform 1 (NP_000469.3) only. The immunizing peptide represents the N terminus of the mature protein.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55 kDa band observed in Human Kidney, Lung and Adrenal Gland lysates (calculated MW of 57.3 kDa according to NP_000469.3). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ALPL

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Alternative Name: ALPL

Background: ALPL, alkaline phosphatase, liver/bone/kidney, AP-TNAP, APTNAP, HOPS, TNAP, TNSALP, alkaline phosphatase liver/bone/kidney isozyme, alkaline phosphatase, tissue-nonspecific isozyme, alkaline phosphomonoesterase, glycerophosphatase, liver/bone/kidney-type

Gene ID: 249, 11647, 25586

NCBI Accession: NP_000469