DARS antibody (N-Term)

Catalog No. ABIN5539478

This Goat Polyclonal antibody specifically detects DARS in WB and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for DARS antibody (N-Term) (ABIN5539478)

Target: DARS (Aspartyl-tRNA Synthetase (DARS))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This DARS antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-DARS Antibody

Binding Specificity: N-Term

Purpose: Aspartyl-tRNA Synthetase (N terminus)

Sequence: SRKGQEKPRE IVD

Specificity: This antibody is expected to recognize reported isoform 1 (NP_803228.2) only.

Cross-Reactivity: Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55 kDa band observed in Mouse fetal Brain lysates (calculated MW of 57.1 kDa according to NP_803228.2). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DARS

Target: DARS (Aspartyl-tRNA Synthetase (DARS))

Alternative Name: Dars

Background: Dars, aspartyl-tRNA synthetase, 5730439G15Rik, aspRS, aspartyl-tRNA synthetase, cytoplasmic

Gene ID: 226414

NCBI Accession: NP_803228, NP_663482