BMP4 antibody (Internal Region)

Catalog No. ABIN5539481

This Goat Polyclonal antibody specifically detects BMP4 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for BMP4 antibody (Internal Region) (ABIN5539481)

Target: BMP4 (Bone Morphogenetic Protein 4 (BMP4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This BMP4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-BMP4 Antibody

Binding Specificity: Internal Region

Purpose: BMP4

Sequence: SPKHHSQRAR K

Specificity: The immunizing peptide represents the N terminus of the mature protein. Reported variants represent identical protein: NP_570911.2, NP_001193.2, NP_570912.2.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 40-45 kDa band observed in lysates of cell lines A431 and HeLa (calculated MW of 46.6 kDa according to NP_001193.2). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for BMP4

Target: BMP4 (Bone Morphogenetic Protein 4 (BMP4))

Alternative Name: BMP4

Background: BMP4, bone morphogenetic protein 4, BMP2B, BMP2B1, MCOPS6, OFC11, ZYME, BMP-2B, BMP-4, bone morphogenetic protein 2B

Gene ID: 652

NCBI Accession: NP_001193

Pathways: Steroid Hormone Mediated Signaling Pathway, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development