CD28 antibody (Internal Region)

Catalog No. ABIN5539489

This anti-CD28 antibody is a Goat Polyclonal antibody detecting CD28 in WB and ELISA. Suitable for Mouse.

Quick Overview for CD28 antibody (Internal Region) (ABIN5539489)

Target: CD28

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-CD28 Antibody

Binding Specificity: Internal Region

Purpose: Cd28 (mouse)

Sequence: KEKHLCHTQS SPK

Specificity: The immunizing peptide represents a part of the extracellular domain.

Cross-Reactivity: Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 40 kDa band observed in Mouse Thymus lysates (calculated MW of 25.2 kDa according to NP_031668.3). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CD28

Target: CD28

Alternative Name: Cd28

Background: Cd28, CD28 antigen, CH29-189K17.3, OTTMUSP00000002157, T-cell-specific surface glycoprotein CD28

Gene ID: 12487

NCBI Accession: NP_031668

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response