Integrin alpha 1 antibody (Internal Region)

Catalog No. ABIN5539494

The Goat Polyclonal anti-Integrin alpha 1 antibody has been validated for WB and ELISA. It is suitable to detect Integrin alpha 1 in samples from Human.

Quick Overview for Integrin alpha 1 antibody (Internal Region) (ABIN5539494)

Target: Integrin alpha 1 (ITGA1)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Integrin alpha 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Integrin alpha 1 Antibody

Binding Specificity: Internal Region

Purpose: ITGA1 / VLA1

Sequence: DKHDFQDSVR IT

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 150 kDa band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 131 kDa according to NP_852478.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Integrin alpha 1

Target: Integrin alpha 1 (ITGA1)

Alternative Name: ITGA1

Background: ITGA1, integrin, alpha 1, CD49a, VLA1, CD49 antigen-like family member A, VLA-1, integrin alpha-1, laminin and collagen receptor, very late activation protein 1

Gene ID: 3672

NCBI Accession: NP_852478

Pathways: EGFR Signaling Pathway, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex