Contactin 1 antibody (Internal Region)

Catalog No. ABIN5539512

The Goat Polyclonal anti-Contactin 1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect Contactin 1 in samples from Human, Rat, Mouse and Pig.

Quick Overview for Contactin 1 antibody (Internal Region) (ABIN5539512)

Target: Contactin 1 (CNTN1)

Reactivity: Human, Rat, Mouse, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This Contactin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-Contactin 1 Antibody

Binding Specificity: Internal Region

Purpose: contactin 1

Sequence: ENIRGKDKHQ AR

Specificity: This antibody is expected to recognize all reported isoforms (NP_001834.2, NP_778203.1, NP_001242992.1). Reported variants represent identical protein: NP_001242993.1, NP_001242992.1.

Cross-Reactivity: Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5 μg/mL.

Western Blot: Approx 140 kDa band observed in Human, Mouse, Rat and Pig Brain lysates (calculated MW of 113 kDa according to NP_001834.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1 μg/mL. Primary in

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Contactin 1

Target: Contactin 1 (CNTN1)

Alternative Name: CNTN1

Background: CNTN1, contactin 1, F3, GP135, contactin-1, glycoprotein gP135, neural cell surface protein F3

Gene ID: 1272, 12805, 117258

NCBI Accession: NP_001834, NP_778203, NP_001242992