EGFR antibody (Internal Region)
Quick Overview for EGFR antibody (Internal Region) (ABIN5539519)
Target
See all EGFR AntibodiesReactivity
Host
Clonality
Conjugate
Application
Grade
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Binding Specificity
- Internal Region
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Purpose
- EGFR
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Sequence
- QKGSHQISLD NPD
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Specificity
- This antibody is expected to recognise isoform a (NP_005219.2) only.
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Cross-Reactivity
- Human
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Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Isotype
- IgG
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anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibody
EGFR Reactivity: Human WB, IHC, ELISA, FACS Host: Mouse Monoclonal 7A6F12 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 888-1210) antibodyEGFR Reactivity: Human WB, IHC Host: Mouse Monoclonal C4 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (pSer1071) antibodyEGFR Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (pSer1026) antibodyEGFR Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (pSer695) antibodyEGFR Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 1163-1191), (C-Term) antibodyEGFR Reactivity: Human WB, IHC (p) Host: Mouse Monoclonal 688CT33-1-3 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (C-Term) antibodyEGFR Reactivity: Human, Mouse, Rat, Monkey, Pig, Cow, Chicken, Dog, Horse, Rabbit, Guinea Pig, Avian, Cat, Donkey, Goat, Hamster, Sheep WB Host: Goat Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibodyEGFR Reactivity: Human WB, ELISA, FACS Host: Mouse Monoclonal 5E10D3 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (C-Term) antibodyEGFR Reactivity: Human, Rat WB, IHC, ELISA, IP Host: Rabbit Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 888-1210) antibodyEGFR Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
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Application Notes
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Western Blot: Approx 170 kDa band observed in lysates of cell line HeLa (calculated MW of 134 kDa according to NP_005219.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.1-0.3 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:128000.
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Comment
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Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Minimize freezing and thawing.
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Storage
- -20 °C
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Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- EGFR (Epidermal Growth Factor Receptor (EGFR))
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Alternative Name
- EGFR
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Background
- EGFR, epidermal growth factor receptor, erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian , HGNC:3236, ERBB, ERBB1, mENA , avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, epidermal growth factor receptor, epidermal growth fac
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Gene ID
- 1956
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NCBI Accession
- NP_005219
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Pathways
- NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins
Target
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