EGFR antibody (Internal Region)

Catalog No. ABIN5539519

This anti-EGFR antibody is a Goat Polyclonal antibody detecting EGFR in WB and ELISA. Suitable for Human.

Quick Overview for EGFR antibody (Internal Region) (ABIN5539519)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This EGFR antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-EGFR Antibody

Binding Specificity: Internal Region

Purpose: EGFR

Sequence: QKGSHQISLD NPD

Specificity: This antibody is expected to recognise isoform a (NP_005219.2) only.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 170 kDa band observed in lysates of cell line HeLa (calculated MW of 134 kDa according to NP_005219.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: EGFR

Background: EGFR, epidermal growth factor receptor, erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian , HGNC:3236, ERBB, ERBB1, mENA , avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, epidermal growth factor receptor, epidermal growth fac

Gene ID: 1956

NCBI Accession: NP_005219

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins