Flotillin 1 antibody (C-Term) (Biotin)

Catalog No. ABIN5539526

This Goat Polyclonal antibody specifically detects Flotillin 1 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for Flotillin 1 antibody (C-Term) (Biotin) (ABIN5539526)

Target: Flotillin 1 (FLOT1)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Flotillin 1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-Flotillin 1 Antibody (Biotin)

Binding Specificity: C-Term

Purpose: Flotillin 1, Biotinylated

Sequence: CSISQVNHKP LRTA.

Specificity: This antibody is expected to recognize both reported isoforms (NP_005794.1, NP_001305804.1).

Cross-Reactivity: Cow, Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 48 kDa band observed in lysates of cell line K562 (calculated MW of 47.4 kDa according to NP_005794.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:2000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Flotillin 1

Target: Flotillin 1 (FLOT1)

Alternative Name: FLOT1

Background: FLOT1, flotillin 1, integral membrane component of caveolae

Gene ID: 10211

NCBI Accession: NP_005794, NP_001305804