GPC1 antibody (Internal Region)

Catalog No. ABIN5539533

The Goat Polyclonal anti-GPC1 antibody has been validated for WB and ELISA. It is suitable to detect GPC1 in samples from Human.

Quick Overview for GPC1 antibody (Internal Region) (ABIN5539533)

Target: GPC1 (Glypican 1 (GPC1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GPC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-GPC1 Antibody

Binding Specificity: Internal Region

Purpose: Glypican-1

Sequence: TDKFWGTSGV ES

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60 kDa band observed in lysates of cell lines HeLa and K562 (calculated MW of 61.7 kDa according to NP_002072.2). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GPC1

Target: GPC1 (Glypican 1 (GPC1))

Alternative Name: GPC1

Background: GPC1, glypican 1, glypican, glypican proteoglycan 1, glypican-1

Gene ID: 2817

NCBI Accession: NP_002072

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation