HDAC3 antibody (Internal Region)

Catalog No. ABIN5539535

This Goat Polyclonal antibody specifically detects HDAC3 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for HDAC3 antibody (Internal Region) (ABIN5539535)

Target: HDAC3 (Histone Deacetylase 3 (HDAC3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HDAC3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-HDAC3 Antibody

Binding Specificity: Internal Region

Purpose: HDAC3

Sequence: NHAPSVQIHD VPAD

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 50 kDa band observed in nuclear lysates of cell line Jurkat (calculated MW of 48.8 kDa according to NP_003874.2). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HDAC3

Target: HDAC3 (Histone Deacetylase 3 (HDAC3))

Alternative Name: HDAC3

Background: HDAC3, histone deacetylase 3, HD3, RPD3, RPD3-2, SMAP45

Gene ID: 8841, 15183, 84578

NCBI Accession: NP_003874

Pathways: Neurotrophin Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development