HMBS antibody (AA 160-173)

Catalog No. ABIN5539536

The Goat Polyclonal anti-HMBS antibody has been validated for WB, ELISA and IHC. It is suitable to detect HMBS in samples from Human.

Quick Overview for HMBS antibody (AA 160-173) (ABIN5539536)

Target: HMBS (Hydroxymethylbilane Synthase (HMBS))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HMBS antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-HMBS Antibody

Binding Specificity: AA 160-173

Purpose: HMBS (aa160-173)

Sequence: HLEFRSIRGN LNTR

Specificity: This antibody is expected to recognize all reported isoforms (NP_000181.2, NP_001019553.1, NP_001245137.1, NP_001245138.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5 μg/mL.

Western Blot: Approx 40 kDa band observed in lysates of cell line K562 (calculated MW of 39.3 kDa according to NP_000181.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HMBS

Target: HMBS (Hydroxymethylbilane Synthase (HMBS))

Alternative Name: HMBS

Background: HMBS, hydroxymethylbilane synthase, PBG-D, PBGD, PORC, UPS, porphobilinogen deaminase, porphyria, acute, Chester type, pre-uroporphyrinogen synthase, uroporphyrinogen I synthase, uroporphyrinogen I synthetase

Gene ID: 3145

NCBI Accession: NP_000181, NP_001019553, NP_001245137, NP_001245138