HRH3 antibody (Internal Region)

Catalog No. ABIN5539537

The Goat Polyclonal anti-HRH3 antibody (ABIN5539537) specifically detects HRH3 in WB, ELISA and IHC. The antibody is reactive with Human samples.

Quick Overview for HRH3 antibody (Internal Region) (ABIN5539537)

Target: HRH3 (Histamine Receptor H3 (HRH3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HRH3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-HRH3 Antibody

Binding Specificity: Internal Region

Purpose: histamine H3 receptor

Sequence: SVTRAVSYRA QQGDT

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 5 μg/mL.

Western Blot: Approx 48 kDa band observed in lysates of cell line A549 (calculated MW of 48.7 kDa according to NP_009163.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HRH3

Target: HRH3 (Histamine Receptor H3 (HRH3))

Alternative Name: HRH3

Background: HRH3, histamine receptor H3, GPCR97, HH3R, G protein-coupled receptor 97, G-protein coupled receptor 97, H3R, OTTHUMP00000031459, histamine H3 receptor

Gene ID: 11255, 99296, 85268

NCBI Accession: NP_009163

Pathways: cAMP Metabolic Process, Feeding Behaviour