LIMK2 antibody (AA 181-193)

Catalog No. ABIN5539550

This Goat Polyclonal antibody specifically detects LIMK2 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for LIMK2 antibody (AA 181-193) (ABIN5539550)

Target: LIMK2 (LIM Domain Kinase 2 (LIMK2))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This LIMK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-LIMK2 Antibody

Binding Specificity: AA 181-193

Purpose: LIMK2 (aa181-193)

Sequence: QVKEVNRMHI SPN

Specificity: This antibody is expected to recognize all reported isoforms (NP_001026971.1, NP_005560.1, NP_057952.1).

Cross-Reactivity: Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 75+80 kDa doublet band observed in lysates of cell lines HeLa and NIH3T3 and approx 70 kDa observed in Rat Pancreas lysates (calculated MW of 77.9 kDa according to Human NP_001026971.1 and 72.2 kDa according to Human NP_005560.1, Mouse N

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for LIMK2

Target: LIMK2 (LIM Domain Kinase 2 (LIMK2))

Alternative Name: LIMK2

Background: LIMK2, LIM domain kinase 2

Gene ID: 3985, 16886, 29524

NCBI Accession: NP_001026971, NP_005560, NP_057952