Lamin B1 antibody (AA 526-537)

Catalog No. ABIN5539551

The Goat Polyclonal anti-Lamin B1 antibody is suitable to detect Lamin B1 in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for Lamin B1 antibody (AA 526-537) (ABIN5539551)

Target: Lamin B1 (LMNB1)

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Lamin B1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Lamin B1 Antibody

Binding Specificity: AA 526-537

Purpose: Lamin B1 (aa526-537)

Sequence: DVKVILKNSQ GE

Specificity: This antibody is expected to recognize both reported isoforms (NP_005564.1, NP_001185486.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70 kDa band observed in nuclear lysates of cell lines HeLa and Jurkat and approx. 70-75 kDa in Mouse Liver lysates (calculated MW of 66.4 kDa according to Human NP_005564.1 and 66.8 kDa according to Mouse NP_034851.2) Recommended concen

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Lamin B1

Target: Lamin B1 (LMNB1)

Alternative Name: LMNB1

Background: LMNB1, lamin B1, ADLD, LMN, LMN2, LMNB, MGC111419, OTTHUMP00000159218, lamin-B1

Gene ID: 4001, 16906

NCBI Accession: NP_005564, NP_001185486

Pathways: Apoptosis, Caspase Cascade in Apoptosis