Muscarinic Acetylcholine Receptor M2 antibody (AA 352-364)

Catalog No. ABIN5539560

The Goat Polyclonal anti-Muscarinic Acetylcholine Receptor M2 antibody has been validated for WB and ELISA. It is suitable to detect Muscarinic Acetylcholine Receptor M2 in samples from Mouse.

Quick Overview for Muscarinic Acetylcholine Receptor M2 antibody (AA 352-364) (ABIN5539560)

Target: Muscarinic Acetylcholine Receptor M2 (CHRM2) (Cholinergic Receptor, Muscarinic 2 (CHRM2))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Muscarinic Acetylcholine Receptor M2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Muscarinic Acetylcholine Receptor M2 Antibody

Binding Specificity: AA 352-364

Purpose: CHRM2 (aa352-364)

Sequence: QNGDEKQNIV ARK

Specificity: Reported variants represent identical protein: NP_001006628.1, NP_001006633.1, NP_001006627.1, NP_000730.1, NP_001006629.1, NP_001006630.1, NP_001006631.1, NP_001006632.1.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 50 kDa band observed in Mouse Brain lysates (calculated MW of 51.5 kDa according to Mouse NP_987076.2). Recommended concentration: 0.5-2 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Muscarinic Acetylcholine Receptor M2

Target: Muscarinic Acetylcholine Receptor M2 (CHRM2) (Cholinergic Receptor, Muscarinic 2 (CHRM2))

Alternative Name: CHRM2

Background: CHRM2, cholinergic receptor, muscarinic 2, FLJ43243, HM2, MGC120006, MGC120007, 7TM receptor, OTTHUMP00000208393, OTTHUMP00000208394, OTTHUMP00000208395, OTTHUMP00000208396, muscarinic M2 receptor, muscarinic acetylcholine receptor M2

Gene ID: 1129, 243764

NCBI Accession: NP_000730