SP1 antibody (Internal Region)

Catalog No. ABIN5539594

The Goat Polyclonal anti-SP1 antibody (ABIN5539594) specifically detects SP1 in WB and ELISA. The antibody is reactive with Mouse samples.

Quick Overview for SP1 antibody (Internal Region) (ABIN5539594)

Target: SP1 (Sp1 Transcription Factor (SP1))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This SP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-SP1 Antibody

Binding Specificity: Internal Region

Purpose: Sp1(mouse)

Sequence: DGIHDETAGG EGEN

Cross-Reactivity: Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 100 kDa band observed in nuclear lysates of cell line NIH3T3 (calculated MW of 80.4 kDa according to NP_038700.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SP1

Target: SP1 (Sp1 Transcription Factor (SP1))

Alternative Name: Sp1

Background: Sp1, trans-acting transcription factor 1, 1110003E12Rik, AA450830, AI845540, Sp1-1

Gene ID: 20683

NCBI Accession: NP_038700

Pathways: Regulation of Lipid Metabolism by PPARalpha, Myometrial Relaxation and Contraction