Thyroperoxidase antibody (Internal Region) (Biotin)

Catalog No. ABIN5539601

This Goat Polyclonal antibody specifically detects Thyroperoxidase in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Thyroperoxidase antibody (Internal Region) (Biotin) (ABIN5539601)

Target: Thyroperoxidase (TPO) (Thyroid Peroxidase (TPO))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Thyroperoxidase antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Thyroperoxidase Antibody (Biotin)

Binding Specificity: Internal Region

Purpose: Thyroid peroxidase, Biotinylated

Sequence: CTRHVIQVSN EVVTDD.

Specificity: This antibody is expected to recognize reported isoforms a (NP_000538.3), b (NP_783650.1), d (NP_78652.1), and e (NP_783653.1). Reported variants represent identical protein: NP_000538.3, NP_001193673.1 Reported variants represent identical protein: NP_78

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 110 kDa band observed in Human Thyroid Gland lysates (calculated MW of 103 kDa according to NP_000538.3). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Thyroperoxidase

Target: Thyroperoxidase (TPO) (Thyroid Peroxidase (TPO))

Alternative Name: Thyroid Peroxidase

Background: TPO, thyroid peroxidase, MSA, TDH2A, TPX, thyroid microsomal antigen, thyroperoxidase

Gene ID: 7173

NCBI Accession: NP_000538, NP_783650, NP_783652, NP_783653

Pathways: Thyroid Hormone Synthesis