Myotilin antibody (AA 209-221)

Catalog No. ABIN5539630

This anti-Myotilin antibody is a Goat Polyclonal antibody detecting Myotilin in WB and ELISA. Suitable for Human.

Quick Overview for Myotilin antibody (AA 209-221) (ABIN5539630)

Target: Myotilin (MYOT)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Myotilin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Myotilin Antibody

Binding Specificity: AA 209-221

Purpose: myotilin (aa209-221)

Sequence: DSQQHNSEHA RLQ

Specificity: This antibody is expected to recognize both reported isoforms (NP_006781.1, NP_001129412.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60 kDa band observed in Human Skeletal Muscle lysates (calculated MW of 55.4 kDa according to NP_006781.1. Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Myotilin

Target: Myotilin (MYOT)

Alternative Name: MYOT

Background: MYOT, myotilin, LGMD1, LGMD1A, TTID, 57 kDa cytoskeletal protein, OTTHUMP00000159430, OTTHUMP00000223460, OTTHUMP00000223461, myofibrillar titin-like Ig domains protein, titin immunoglobulin domain protein (myotilin)

Gene ID: 9499

NCBI Accession: NP_006781, NP_001129412