REST antibody (Internal Region)

Catalog No. ABIN5539667

This Goat Polyclonal antibody specifically detects REST in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for REST antibody (Internal Region) (ABIN5539667)

Target: REST (RE1-Silencing Transcription Factor (REST))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This REST antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-REST Antibody

Binding Specificity: Internal Region

Purpose: RE1-silencing transcription factor

Sequence: PETPGAEDKG KSSK

Specificity: Reported variants represent identical protein: NP_001180437.1, NP_005603.3

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 140 kDa band observed in nuclear lysates of cell lines HEK293 and HeLa (calculated MW of 122 kDa according to NP_005603.3). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for REST

Target: REST (RE1-Silencing Transcription Factor (REST))

Alternative Name: REST

Background: REST, RE1-silencing transcription factor, NRSF, XBR, RE1-silencing transcription factor variant E1a/E2/E3/E4c, RE1-silencing transcription factor variant E1a/E2/E3/E5, RE1-silencing transcription factor variant E1a/E2/E3/N3a/E4i, RE1-silencing transcripti

Gene ID: 5978

NCBI Accession: NP_005603

Pathways: Negative Regulation of Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Chromatin Binding, Positive Regulation of Endopeptidase Activity