HMGCS1 antibody (AA 321-332)

Catalog No. ABIN5539672

This anti-HMGCS1 antibody is a Goat Polyclonal antibody detecting HMGCS1 in WB and ELISA. Suitable for Human.

Quick Overview for HMGCS1 antibody (AA 321-332) (ABIN5539672)

Target: HMGCS1 (3-Hydroxy-3-Methylglutaryl-CoA Synthase 1 (Soluble) (HMGCS1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HMGCS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-HMGCS1 Antibody

Binding Specificity: AA 321-332

Purpose: HMGCS1 (aa321-332)

Sequence: KASSELFSQK TK

Specificity: Reported variants represent identical protein: NP_002121.4, NP_001091742.1.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 57 kDa band observed in lysates of cell lines HeLa, HepG2, and K562 (calculated MW of 57.3 kDa according to NP_002121.4). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HMGCS1

Target: HMGCS1 (3-Hydroxy-3-Methylglutaryl-CoA Synthase 1 (Soluble) (HMGCS1))

Alternative Name: HMGCS1

Background: HMGCS1, 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble), HMGCS, 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase, 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble), hydroxymethylglutaryl-CoA synthase, cytoplasmic

Gene ID: 3157

NCBI Accession: NP_002121

Pathways: Regulation of Lipid Metabolism by PPARalpha