PD-L1 antibody (C-Term) (Biotin)

Catalog No. ABIN5539689

The Goat Polyclonal anti-PD-L1 antibody has been validated for WB and ELISA. It is suitable to detect PD-L1 in samples from Human.

Quick Overview for PD-L1 antibody (C-Term) (Biotin) (ABIN5539689)

Target: PD-L1 (CD274 (PD-L1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PD-L1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-PD-L1 Antibody (Biotin)

Binding Specificity: C-Term

Purpose: CD274 / PD-L1, Biotinylated

Sequence: CKKQSDTHLE ET.

Specificity: This antibody is expected to recognize reported isoforms a and b (NP_054862.1, NP_001254635.1) only.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 40 kDa band observed in Human Heart lysates (calculated MW of 33.3 kDa according to NP_054862.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: CD274

Background: CD274, CD274 Molecule, B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, B7 homolog 1, CD274 antigen, PDCD1 ligand 1

Gene ID: 29126

NCBI Accession: NP_054862, NP_001254635

Pathways: Cancer Immune Checkpoints