CD247 antibody (Internal Region)

Catalog No. ABIN5539696

The Goat Polyclonal anti-CD247 antibody (ABIN5539696) specifically detects CD247 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for CD247 antibody (Internal Region) (ABIN5539696)

Target: CD247 (CD247 Molecule (CD247))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CD247 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-CD247 Antibody

Binding Specificity: Internal Region

Purpose: CD3-ZETA / CD247

Sequence: KNPQEGLYNE LQKD

Specificity: This antibody is expected to recognize both reported isoforms (NP_932170.1, NP_000725.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 16-17 kDa band observed in lysates of cell lines Jurkat and MOLT4 (calculated MW of 18.6 kDa according to NP_000725.1). Recommended concentration: 0.1-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CD247

Target: CD247 (CD247 Molecule (CD247))

Alternative Name: CD247

Background: CD247, CD247 Molecule, CD3-ZETA, CD3H, CD3Q, CD3Z, T3Z, TCRZ, CD247 antigen, zeta subunit, CD3Z antigen, zeta polypeptide (TiT3 complex), CD3zeta chain, T-cell antigen receptor complex, zeta subunit of CD3, T-cell receptor T3 zeta chain, T-cell surface gl

Gene ID: 919

NCBI Accession: NP_932170, NP_000725

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway