CD3 epsilon antibody (Internal Region)

Catalog No. ABIN5539697

This Goat Polyclonal antibody specifically detects CD3 epsilon in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for CD3 epsilon antibody (Internal Region) (ABIN5539697)

Target: CD3 epsilon (CD3E)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-CD3 epsilon Antibody

Binding Specificity: Internal Region

Purpose: CD3E (cytoplasmic)

Sequence: RKGQRDLYSG LNQR

Specificity: This antibody is designed to recognize the cytoplasmic domain (aa153-207) of the protein.

Cross-Reactivity: Human, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 22 kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 23.1 kDa according to NP_000724.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3E

Background: CD3E, CD3e molecule, epsilon (CD3-TCR complex), IMD18, T3E, TCRE, CD3-epsilon, CD3e antigen, epsilon polypeptide (TiT3 complex), T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycopro

Gene ID: 916, 315609

NCBI Accession: NP_000724

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway