KRT20 antibody (AA 48-60)

Catalog No. ABIN5539708

This anti-KRT20 antibody is a Goat Polyclonal antibody detecting KRT20 in WB, IHC and ELISA. Suitable for Human.

Quick Overview for KRT20 antibody (AA 48-60) (ABIN5539708)

Target: KRT20 (Keratin 20 (KRT20))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This KRT20 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-KRT20 Antibody

Binding Specificity: AA 48-60

Purpose: cytokeratin 20 (aa48-60)

Sequence: NSRHTVNYGS DLT

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Colon. Recommended concentration: 5 μg/mL.

Western Blot: Approx 49 kDa band observed in Human Colon and Ileum lysates (calculated MW of 48.5 kDa according to NP_061883.1). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for KRT20

Target: KRT20 (Keratin 20 (KRT20))

Alternative Name: KRT20

Background: KRT20, keratin 20, CK-20, CK20, K20, KRT21, MGC35423, OTTHUMP00000164518, cytokeratin 20, cytokeratin-20, keratin, type I cytoskeletal 20, keratin-20, protein IT

Gene ID: 54474

NCBI Accession: NP_061883