SGCD antibody (AA 245-257)

Catalog No. ABIN5539715

This Goat Polyclonal antibody specifically detects SGCD in WB, IHC and ELISA. It exhibits reactivity toward Human and Rat.

Quick Overview for SGCD antibody (AA 245-257) (ABIN5539715)

Target: SGCD (Sarcoglycan, delta (35kDa Dystrophin-Associated Glycoprotein) (SGCD))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This SGCD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-SGCD Antibody

Binding Specificity: AA 245-257

Purpose: Delta-Sarcoglycan (aa245-257)

Sequence: RLPHGSYTPT GTR

Specificity: This antibody is expected to recognize isoform 1 (NP_000328.2) and isoform 3 (NP_001121681.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Skeletal Muscle shows sarcolemmar staining of the fibres. Recommended concentration, 3-5 μg/mL.

Western Blot: Approx 37 kDa band observed in Human and Rat Heart lysates (calculated MW of 32.2 kDa according to NP_000328.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SGCD

Target: SGCD (Sarcoglycan, delta (35kDa Dystrophin-Associated Glycoprotein) (SGCD))

Alternative Name: SGCD

Background: SGCD, sarcoglycan, delta (35 kDa dystrophin-associated glycoprotein), 35DAG, CMD1L, DAGD, MGC22567, SG-delta, SGCDP, SGD, 35 kDa dystrophin-associated glycoprotein, 35kD dystrophin-associated glycoprotein, delta-SG, delta-sarcoglycan, dystrophin associated

Gene ID: 6444, 24052, 497892

NCBI Accession: NP_000328, NP_758447, NP_001121681

Pathways: Maintenance of Protein Location