Fibrillarin antibody (AA 99-110)

Catalog No. ABIN5539729

This anti-Fibrillarin antibody is a Goat Polyclonal antibody detecting Fibrillarin in WB and ELISA. Suitable for Human.

Quick Overview for Fibrillarin antibody (AA 99-110) (ABIN5539729)

Target: Fibrillarin (FBL)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Fibrillarin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Fibrillarin Antibody

Binding Specificity: AA 99-110

Purpose: fibrillarin / FBL (aa99-110)

Sequence: CRGKEDALVT KN

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 35 kDa band observed in lysates of cell line HEK293 (calculated MW of 33.8 kDa according to NP_001427.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Fibrillarin

Target: Fibrillarin (FBL)

Alternative Name: FBL

Background: FBL, fibrillarin, FIB, FLRN, RNU3IP1, 34 kDa nucleolar scleroderma antigen, 34-kD nucleolar scleroderma antigen, RNA, U3 small nucleolar interacting protein 1, rRNA 2'-O-methyltransferase fibrillarin

Gene ID: 2091, 14113, 292747

NCBI Accession: NP_001427

Pathways: Ribonucleoside Biosynthetic Process