LCAT antibody (AA 366-378)

Catalog No. ABIN5539740

The Goat Polyclonal anti-LCAT antibody has been validated for WB, IHC and ELISA. It is suitable to detect LCAT in samples from Human.

Quick Overview for LCAT antibody (AA 366-378) (ABIN5539740)

Target: LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This LCAT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-LCAT Antibody

Binding Specificity: AA 366-378

Purpose: LCAT (aa366-378)

Sequence: EDGDDTVATR STE

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Liver. Recommended concentration: 5 μg/mL.

Western Blot: Approx 55 kDa band observed in Human Testis lysates (calculated MW of 49.6 kDa according to NP_000220.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room t

Peptide ELISA: antibody detection limit dilution 1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for LCAT

Target: LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))

Alternative Name: LCAT

Background: LCAT, lecithin-cholesterol acyltransferase, phosphatidylcholine-sterol acyltransferase, phospholipid-cholesterol acyltransferase

Gene ID: 3931, 16816, 24530

NCBI Accession: NP_000220

Pathways: Lipid Metabolism