ENC1 antibody (Internal Region)

Catalog No. ABIN5539762

The Goat Polyclonal anti-ENC1 antibody (ABIN5539762) specifically detects ENC1 in ELISA, WB and IHC. The antibody is reactive with Human and Rat samples.

Quick Overview for ENC1 antibody (Internal Region) (ABIN5539762)

Target: ENC1 (Ectodermal-Neural Cortex 1 (With BTB-Like Domain) (ENC1))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This ENC1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-ENC1 Antibody

Binding Specificity: Internal Region

Purpose: ENC1

Sequence: TSVSHDKLPK VQC

Specificity: This antibody is expected to recognize both reported isoforms (NP_003624.1, NP_001243505.1). Reported variants represent identical protein: NP_001243503.1, NP_001243504.1, NP_003624.1.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Adrenal Gland. Recommended concentration: 5 μg/mL.

Western Blot: Approx 70 kDa band observed in Rat Ovary lysates (calculated MW of 66.1 kDa according to Rat NP_001003401.1. Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse Brain for this par

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ENC1

Target: ENC1 (Ectodermal-Neural Cortex 1 (With BTB-Like Domain) (ENC1))

Alternative Name: ENC1

Background: ENC1, ectodermal-neural cortex 1 (with BTB-like domain), CCL28, ENC-1, FLJ39259, KLHL35, KLHL37, NRPB, PIG10, TP53I10, OTTHUMP00000128235, OTTHUMP00000222054, ectoderm-neural cortex protein 1, ectodermal-neural cortex (with BTB-like domain), kelch-like 35

Gene ID: 8507, 13803, 294674

NCBI Accession: NP_003624, NP_001243505