DARC antibody (N-Term) (Biotin)

Catalog No. ABIN5539768

This anti-DARC antibody is a Goat Polyclonal antibody detecting DARC in WB, ELISA, IHC and FACS. Suitable for Human.

Quick Overview for DARC antibody (N-Term) (Biotin) (ABIN5539768)

Target: DARC (Duffy Blood Group, Chemokine Receptor (DARC))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DARC antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-DARC Antibody (Biotin)

Binding Specificity: N-Term

Purpose: Duffy / FY / DARC, Biotinylated

Sequence: HRAELSPSTE NSSQLDFEDC .

Specificity: This antibody is expected to recognize both reported isoforms (NP_001116423.1, NP_002027.2).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 37 kDa band observed in Human Liver lysates (calculated MW of 35.7 kDa according to NP_001116423.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DARC

Target: DARC (Duffy Blood Group, Chemokine Receptor (DARC))

Alternative Name: ACKR1

Background: ACKR1, atypical chemokine receptor 1 (Duffy blood group), CCBP1, CD234, DARC, Dfy, FY, GPD, GpFy, WBCQ1, Duffy antigen chemokine receptor, Duffy blood group antigen, Duffy blood group, atypical chemokine receptor, Duffy blood group, chemokine receptor, Fy

Gene ID: 2532

NCBI Accession: NP_001116423, NP_002027