PARP12 antibody (Internal Region)

Catalog No. ABIN5539770

The Goat Polyclonal anti-PARP12 antibody is suitable to detect PARP12 in samples from Human. It has been validated for ELISA and IF.

Quick Overview for PARP12 antibody (Internal Region) (ABIN5539770)

Target: PARP12 (Poly (ADP-Ribose) Polymerase Family, Member 12 (PARP12))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PARP12 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-PARP12 Antibody

Binding Specificity: Internal Region

Purpose: PARP12

Sequence: NAHDIKNKSS AP

Cross-Reactivity: Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments gave an approx 110 kDa band in Human Liver lysates and in lysates of hepatoblastoma cell line HEPG2 after 0.03 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Anonymous customer has confirmed specificity by siRNA-mediated PARP2 knockdown.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PARP12

Target: PARP12 (Poly (ADP-Ribose) Polymerase Family, Member 12 (PARP12))

Alternative Name: PARP12

Background: PARP12, poly (ADP-ribose) polymerase family, member 12, HGNC:21919, FLJ22693, PARP-12, ZC3H1, ZC3HDC1, zinc finger CCCH type domain containing 1, zinc finger CCCH-type domain containing 1

Gene ID: 64761

NCBI Accession: NP_073587