CD3EAP antibody (Internal Region)
Quick Overview for CD3EAP antibody (Internal Region) (ABIN5539771)
Target
See all CD3EAP AntibodiesReactivity
Host
Clonality
Conjugate
Application
Grade
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Binding Specificity
- Internal Region
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Purpose
- CD3EAP
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Sequence
- PEDKTVKQEQ INTEP
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Cross-Reactivity
- Human
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Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Isotype
- IgG
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Application Notes
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Western Blot: Preliminary experiments in nuclear lysates of cell lines HeLa, Jurkat and A431 gave no specific signal but low background (at antibody concentration up to 1 μg/mL). We would appreciate any feedback from people in the field - have any results
Peptide ELISA: antibody detection limit dilution 1:4000.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Minimize freezing and thawing.
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Storage
- -20 °C
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Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- CD3EAP (CD3e Molecule, epsilon Associated Protein (CD3EAP))
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Alternative Name
- CD3EAP
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Background
- CD3EAP, CD3e molecule, epsilon associated protein, ASE-1, ASE1, CAST, PAF49, A34.5, CD3 epsilon associated protein, CD3-epsilon-associated protein, CD3E-associated protein, CD3e antigen, epsilon polypeptide associated protein, DNA-directed RNA polymerase
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Gene ID
- 10849
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NCBI Accession
- NP_036231
Target
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