ALT antibody (AA 391-405)

Catalog No. ABIN5539794

The Goat Polyclonal anti-ALT antibody has been validated for WB and ELISA. It is suitable to detect ALT in samples from Human.

Quick Overview for ALT antibody (AA 391-405) (ABIN5539794)

Target: ALT (Alanine Aminotransferase (ALT))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ALT antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-ALT Antibody

Binding Specificity: AA 391-405

Purpose: ALT1 / GPT1 (aa391-405)

Sequence: KLTEQVFNEA PGISC

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 52 kDa band observed in Human Liver lysates (calculated MW of 54.6 kDa according to NP_005300.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ALT

Target: ALT (Alanine Aminotransferase (ALT))

Alternative Name: GPT

Background: GPT, glutamic-pyruvate transaminase (alanine aminotransferase), AAT1, ALT1, GPT1, GPT 1, alanine aminotransferase 1, glutamate pyruvate transaminase 1, glutamic--alanine transaminase 1, glutamic--pyruvic transaminase 1, glutamic-alanine transaminase 1

Gene ID: 2875

NCBI Accession: NP_005300