Caveolin-1 antibody (Internal Region) (Biotin)

Catalog No. ABIN5539799

This Goat Polyclonal antibody specifically detects Caveolin-1 in WB, ELISA and ICC. It exhibits reactivity toward Human.

Quick Overview for Caveolin-1 antibody (Internal Region) (Biotin) (ABIN5539799)

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Caveolin-1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Grade: Verified

Product Details anti-Caveolin-1 Antibody (Biotin)

Binding Specificity: Internal Region

Purpose: Caveolin 1, Biotinylated

Sequence: DELSEKQVYD AH.

Specificity: This antibody is expected to recognize both reported isoforms (NP_001744.2, NP_001166366.1). Reported variants represent identical protein: NP_001166367.1, NP_001166368.1, NP_001166366.1. No cross-reactivity expected to Caveolin 2 and Caveolin 3

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 22+24 kDa band observed in Human spleen lysates (calculated MW of 20.5 kDa according to NP_001744.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Caveolin-1

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Alternative Name: CAV1

Background: CAV1, caveolin 1, BSCL3, CGL3, LCCNS, MSTP085, PPH3, VIP21, caveolin 1, caveolae protein, 22 kDa, cell growth-inhibiting protein 32

Gene ID: 857, 12389, 25404

NCBI Accession: NP_001744, NP_001166366

Pathways: Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of Transporter Activity, VEGFR1 Specific Signals