CALB1 antibody (Internal Region)

Catalog No. ABIN5539806

The Goat Polyclonal anti-CALB1 antibody has been validated for IHC, WB and ELISA. It is suitable to detect CALB1 in samples from Human and Rat.

Quick Overview for CALB1 antibody (Internal Region) (ABIN5539806)

Target: CALB1 (Calbindin (CALB1))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This CALB1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-CALB1 Antibody

Binding Specificity: Internal Region

Purpose: calbindin D28

Sequence: KTFVDQYGQR DDGK

Specificity: Immunizing peptide overlaps EF hand 2, but not any calcium binding site.

Cross-Reactivity: Cow, Dog, Human, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5-7 μg/mL.

Western Blot: Approx. 26 kDa band observed in Human and Rat Kidney lysates and in Rat Brain lysates (calculated MW of 30.0 kDa according to Human NP_004920.1and Rat NP_114190.1). The observed molecular weight is routinely observed by other sources. Recomme

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CALB1

Target: CALB1 (Calbindin (CALB1))

Alternative Name: CALB1

Background: CALB1, calbindin 1, 28 kDa, CALB, D-28K, OTTHUMP00000166027, OTTHUMP00000225441, RTVL-H protein, calbindin, calbindin 1, (28kD), calbindin D28, vitamin D-dependent calcium-binding protein, avian-type

Gene ID: 793, 83839

NCBI Accession: NP_004920