ARF4 antibody (AA 137-150)

Catalog No. ABIN5539807

This anti-ARF4 antibody is a Goat Polyclonal antibody detecting ARF4 in ELISA, WB and IHC. Suitable for Human and Mouse.

Quick Overview for ARF4 antibody (AA 137-150) (ABIN5539807)

Target: ARF4 (ADP-Ribosylation Factor 4 (ARF4))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This ARF4 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-ARF4 Antibody

Binding Specificity: AA 137-150

Purpose: ARF4 (aa137-150)

Sequence: SEMTDKLGLQ SLRN

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Thymus. Recommended concentration: 5 μg/mL.

Western Blot: Approx 19 kDa band observed in lysates of cell lines NIH3T3 and HeLa (calculated MW of 20.5 kDa according to Human NP_001651.1 and 20.4 kDa according to Mouse NP_031505.1 ). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at r

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ARF4

Target: ARF4 (ADP-Ribosylation Factor 4 (ARF4))

Alternative Name: ARF4

Background: ARF4, ADP-ribosylation factor 4, ARF2, ADP-ribosylation factor 2, OTTHUMP00000213140, OTTHUMP00000213142, OTTHUMP00000213211

Gene ID: 378, 11843, 79120

NCBI Accession: NP_001651

Pathways: EGFR Signaling Pathway, Transition Metal Ion Homeostasis